Radioelectronics and radioelectronic equipment manufacturing

Influence of Eddition Buffer Layer on CRP Sensor Sensitivity Based on ISFET

Authors

O. Yu. Kutova
National Technical University of Ukraine "Igor Sikorsky Kyiv Polytechnic Institute"
M. H. Dusheiko
National Technical University of Ukraine "Igor Sikorsky Kyiv Polytechnic Institute"
M. O. Semenenko
V. E. Lashkaryov Institute of Semiconductor Physics NAS of Ukraine, Kyiv
L. A. Stoliar
UkrGenTech, Ltd, Kyiv
V. I. Tymofieiev
National Technical University of Ukraine "Igor Sikorsky Kyiv Polytechnic Institute"
DOI https://doi.org/10.31649/1997-9266-2018-140-5-98-104

Keywords

biosensor С-reactive protein melanin sensitivity

Abstract

The properties of sensor structure based on ion selectivity field effect transistor for electrical detection of C-reactive protein (CRP) were described and investigated in this paper. This protein is an acute phase biomarker that allows identification of inflammation in human body and also the CRP blood level is known to be associated with probability of cardio-vascular diseases. The physico-technlogical and topological characteristics of sensor based on ISFET with СеО2 gate dielectric were studied and described in this paper. Cerium dioxide is a promising material for utilization as a gate oxide for FET fabrication and has a number of advantages compared to SiO2, In particular, it characterized by higher density of surface sensitive centers, better dielectric structure isolation, less current leakage through the dielectric, higher thermal and chemical stability, biocompatibility, as well as effective immobilization of biochemical elements using membranes to create biosensors. Silicon and cerium materials also have close value soflattice constants, ensuring their better adhesion in the structure of the ISFET. The experimental characteristics for both modification of sensors with and without addition buffer layer (melanin) were compared. Surfaces of both structures are almost the same in optical microscope, except surface with melanin has more branched structure. Based on this it was assumed that more streptavidin can be deposited on the surface and, as a result, a greater number of pairs of antigen-antibodies as one of the main working reactions of the biosensors. According to the measurements, sensitivity for both types of sensors was determined and it was established that the sensor sensitivity without melanin was 92 mV/(mg/L), and with addition buffer layer — 204,4 mV/(mg/L). Therefore the additional layer of melanin increases the sensor sensitivity more than 2 folds and this type of sensor is valuable to detect inflammation in human body at the early stage and predict the probability to appear the cardiovascular diseases and also decrease the number of deaths.

430 281

How to Cite

[1]
“Influence of Eddition Buffer Layer on CRP Sensor Sensitivity Based on ISFET”, Вісник ВПІ, no. 5, pp. 98–104, Nov. 2018, doi: 10.31649/1997-9266-2018-140-5-98-104.

Author Biographies

O. Yu. Kutova, National Technical University of Ukraine "Igor Sikorsky Kyiv Polytechnic Institute"

Assistant of the Chair of Microelectronics

M. H. Dusheiko, National Technical University of Ukraine "Igor Sikorsky Kyiv Polytechnic Institute"

Senior Engineer of Scientific Laboratory of Semiconductor of the Chair of Microelectronics

M. O. Semenenko, V. E. Lashkaryov Institute of Semiconductor Physics NAS of Ukraine, Kyiv

Cand. Sc.(Ph.-Math.), Senior Research Assistant of the Laboratory of Physical Bases of Electron Semiconductor Micro- and Nanotechnologies

L. A. Stoliar, UkrGenTech, Ltd, Kyiv

CEO of “UkrGenTech”, Ltd

V. I. Tymofieiev, National Technical University of Ukraine "Igor Sikorsky Kyiv Polytechnic Institute"

Dr. Sc. (Eng.), Professor, Head of the Chair of Electronic Engineering

References

E. Prestegаrd, “The future of point–of–care testing using C-reactive protein – ideal tool for diagnosis, prognosis and therapy management,” Businessbriefing: European endocrine review, 2006, pp. 1-4.
C. H. Selfand, and D. B. Cook, “Advances inimmunoassay technology”, vol. 7, no. 1, pp. 60-65, 1996.
J. B. Aguiara Francisco, et. al., “C-reactive protein: clinical applications and proposals for a rational use,” REV ASSOC MED BRAS. 2013; 59 (1), pp. 85-92.
C. T. Limand, and Y. Zhang, “Bead-based microfluidic immunoassays: the extgeneration,” Biosens Bioelectron, 2007, Feb 15; 22(7), pp. 1197-204.
Y. H. Matthewand, and Ho C. S. Tangaand, “One-step immunoassay of C-reactive protein using droplet microfluidics,” Lab Chip, 16, p. 4359, 2016.
K. R. Katikireddy, and F. O’Sullivan, “Immunohistochemical and immunofluorescence procedures for protein analysis,” Methods Mol Biol, 2011, pp 784:155-67.
C.-L. Lin, Y.-W. Kang, K-W. Chang, W-H. Chang, Y-L. Wangandand, G.-B. Lee., “An integrated microfluidic system using field-effect transistors for CRP detection,” in 28th IEEE International Conference on Micro Electro Mechanical Systems, 18-22 Jan, 2015.
H. C. Longuet-Higgins “On the origin of the free radical property of melanins,” Arch Biochem Biophys, Feb, 86:231-2, 1960.
W.-C. Kao, et. al., “Detection of C-reactive protein on an integrated microfluidic system by utilizing field-effect transistors and aptamers”, Biomicrofluidic, 11, 2017.